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Amaxa Nhdf Nucleofector Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amaxatm Nhdf Nucleofectortm Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lonza human keratinocyte nucleofector kit
Human Keratinocyte Nucleofector Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lonza amaxa human keratinocytes nucleofector kit
( a ) NHEKs were transfected with pUNO-mcs or pUNO-hTLR9-HA using Amaxa Human <t>Keratinocyte</t> <t>Nucleofector</t> Kit. 24 h after the transfection TLR9 mRNA expression were examined. ( b – d ) 24 h after the transfection with pUNO-mcs or pUNO-hTLR9-HA, NHEKs were stimulated with CpG alone (2 μM) ( b ) or LL-37 (10 μM) then CpG (2 μM) (c), genomic DNA (10 μg/ml) ( d ) for 24 h. The expression of IFNB1 mRNA was analyzed by RT-qPCR. *: P < 0.05, **: P < 0.01. Data are mean ± SEM of triplicate samples and representative of two independent experiments.
Amaxa Human Keratinocytes Nucleofector Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltrace+tm+violet+cell+proliferation+kit+%28vpd/pmc03220926-121-5-9?v=Lonza
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Lonza human telomerase-immortalized human dermal lymphatic endothelial cells (htert-hdlecs
( a ) NHEKs were transfected with pUNO-mcs or pUNO-hTLR9-HA using Amaxa Human <t>Keratinocyte</t> <t>Nucleofector</t> Kit. 24 h after the transfection TLR9 mRNA expression were examined. ( b – d ) 24 h after the transfection with pUNO-mcs or pUNO-hTLR9-HA, NHEKs were stimulated with CpG alone (2 μM) ( b ) or LL-37 (10 μM) then CpG (2 μM) (c), genomic DNA (10 μg/ml) ( d ) for 24 h. The expression of IFNB1 mRNA was analyzed by RT-qPCR. *: P < 0.05, **: P < 0.01. Data are mean ± SEM of triplicate samples and representative of two independent experiments.
Human Telomerase Immortalized Human Dermal Lymphatic Endothelial Cells (Htert Hdlecs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FUJIFILM anti dykddddk tag antibody beads
( a ) NHEKs were transfected with pUNO-mcs or pUNO-hTLR9-HA using Amaxa Human <t>Keratinocyte</t> <t>Nucleofector</t> Kit. 24 h after the transfection TLR9 mRNA expression were examined. ( b – d ) 24 h after the transfection with pUNO-mcs or pUNO-hTLR9-HA, NHEKs were stimulated with CpG alone (2 μM) ( b ) or LL-37 (10 μM) then CpG (2 μM) (c), genomic DNA (10 μg/ml) ( d ) for 24 h. The expression of IFNB1 mRNA was analyzed by RT-qPCR. *: P < 0.05, **: P < 0.01. Data are mean ± SEM of triplicate samples and representative of two independent experiments.
Anti Dykddddk Tag Antibody Beads, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lonza amaxa nhdf kit
( a ) NHEKs were transfected with pUNO-mcs or pUNO-hTLR9-HA using Amaxa Human <t>Keratinocyte</t> <t>Nucleofector</t> Kit. 24 h after the transfection TLR9 mRNA expression were examined. ( b – d ) 24 h after the transfection with pUNO-mcs or pUNO-hTLR9-HA, NHEKs were stimulated with CpG alone (2 μM) ( b ) or LL-37 (10 μM) then CpG (2 μM) (c), genomic DNA (10 μg/ml) ( d ) for 24 h. The expression of IFNB1 mRNA was analyzed by RT-qPCR. *: P < 0.05, **: P < 0.01. Data are mean ± SEM of triplicate samples and representative of two independent experiments.
Amaxa Nhdf Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltrace+tm+violet+cell+proliferation+kit+%28vpd/pmc04988562-696-27-29?v=Lonza
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Lonza adult human dermal lecs
( a ) NHEKs were transfected with pUNO-mcs or pUNO-hTLR9-HA using Amaxa Human <t>Keratinocyte</t> <t>Nucleofector</t> Kit. 24 h after the transfection TLR9 mRNA expression were examined. ( b – d ) 24 h after the transfection with pUNO-mcs or pUNO-hTLR9-HA, NHEKs were stimulated with CpG alone (2 μM) ( b ) or LL-37 (10 μM) then CpG (2 μM) (c), genomic DNA (10 μg/ml) ( d ) for 24 h. The expression of IFNB1 mRNA was analyzed by RT-qPCR. *: P < 0.05, **: P < 0.01. Data are mean ± SEM of triplicate samples and representative of two independent experiments.
Adult Human Dermal Lecs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( a ) NHEKs were transfected with pUNO-mcs or pUNO-hTLR9-HA using Amaxa Human Keratinocyte Nucleofector Kit. 24 h after the transfection TLR9 mRNA expression were examined. ( b – d ) 24 h after the transfection with pUNO-mcs or pUNO-hTLR9-HA, NHEKs were stimulated with CpG alone (2 μM) ( b ) or LL-37 (10 μM) then CpG (2 μM) (c), genomic DNA (10 μg/ml) ( d ) for 24 h. The expression of IFNB1 mRNA was analyzed by RT-qPCR. *: P < 0.05, **: P < 0.01. Data are mean ± SEM of triplicate samples and representative of two independent experiments.

Journal: The Journal of investigative dermatology

Article Title: Cathelicidin antimicrobial peptide LL-37 in psoriasis enables keratinocyte reactivity against TLR9 ligands

doi: 10.1038/jid.2011.259

Figure Lengend Snippet: ( a ) NHEKs were transfected with pUNO-mcs or pUNO-hTLR9-HA using Amaxa Human Keratinocyte Nucleofector Kit. 24 h after the transfection TLR9 mRNA expression were examined. ( b – d ) 24 h after the transfection with pUNO-mcs or pUNO-hTLR9-HA, NHEKs were stimulated with CpG alone (2 μM) ( b ) or LL-37 (10 μM) then CpG (2 μM) (c), genomic DNA (10 μg/ml) ( d ) for 24 h. The expression of IFNB1 mRNA was analyzed by RT-qPCR. *: P < 0.05, **: P < 0.01. Data are mean ± SEM of triplicate samples and representative of two independent experiments.

Article Snippet: Transfection was performed by Amaxa Human Keratinocytes Nucleofector Kit (Lonza Cologne AG).

Techniques: Transfection, Expressing, Quantitative RT-PCR